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Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon.

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Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon.

Int J Legal Med. 2014 Nov 19;

Authors: Warshauer DH, Davis CP, Holt C, Han Y, Walichiewicz P, Richardson T, Stephens K, Jager A, King J, Budowle B

Abstract
The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. In this study, the TruSeq™ Forensic Amplicon library preparation protocol was used to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples. Results were compared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS).

PMID: 25408291 [PubMed - as supplied by publisher]

Dual small fragment plating improves screw-to-screw load sharing for mid-diaphyseal humeral fracture fixation: A finite element study.

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Dual small fragment plating improves screw-to-screw load sharing for mid-diaphyseal humeral fracture fixation: A finite element study.

Technol Health Care. 2014 Nov 18;

Authors: Kosmopoulos V, Luedke C, Nana AD

Abstract
BACKGROUND: A smaller humerus in some patients makes the use of a large fragment fixation plate difficult. Dual small fragment plate constructs have been suggested as an alternative.
OBJECTIVE: This study compares the biomechanical performance of three single and one dual plate construct for mid-diaphyseal humeral fracture fixation.
METHODS: Five humeral shaft finite element models (1 intact and 4 fixation) were loaded in torsion, compression, posterior-anterior (PA) bending, and lateral-medial (LM) bending. A comminuted fracture was simulated by a 1-cm gap. Fracture fixation was modelled by: (A) 4.5-mm 9-hole large fragment plate (wide), (B) 4.5-mm 9-hole large fragment plate (narrow), (C) 3.5-mm 9-hole small fragment plate, and (D) one 3.5-mm 9-hole small fragment plate and one 3.5-mm 7-hole small fragment plate.
RESULTS: Model A showed the best outcomes in torsion and PA bending, whereas Model D outperformed the others in compression and LM bending. Stress concentrations were located near and around the unused screw holes for each of the single plate models and at the neck of the screws just below the plates for all the models studied. Other than in PA bending, Model D showed the best overall screw-to-screw load sharing characteristics.
CONCLUSION: The results support using a dual small fragment locking plate construct as an alternative in cases where crutch weight-bearing (compression) tolerance may be important and where anatomy limits the size of the humerus bone segment available for large fragment plate fixation.

PMID: 25408282 [PubMed - as supplied by publisher]

Aberrant Histone Acetylation Promotes Mitochondrial Respiratory Suppression In the Brain of Alcoholic Rats.

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Aberrant Histone Acetylation Promotes Mitochondrial Respiratory Suppression In the Brain of Alcoholic Rats.

J Pharmacol Exp Ther. 2014 Nov 18;

Authors: Jung ME, Metzger DB

Abstract
The acetylation of histone proteins in the core of DNA regulates gene expression, including those affecting mitochondria. Both histone acetylation and mitochondrial deficit have been implicated in neuronal damage associated with drinking problems. Many alcoholics repeat unsuccessful attempts at abstaining, developing a pattern of repeated withdrawal from ethanol exposure. Here, we investigated whether aberrant histone acetylation contributes to mitochondrial and cellular damage induced by repeated ethanol withdrawal (EW). We also investigated whether this effect of histone acetylation involves a small non-coding RNA (microRNA) let-7f. Male rats receive two cycles of ethanol diet (7.5%, four weeks) and withdrawal. Prefrontal cortex was collected to measure mitochondrial respiration and histone acetylation using XF real-time respirometry and gold immunostaining, respectively. Separately, HT22 (mouse hippocampal) cells received two cycles of ethanol exposure (100 mM, 20 hours) and withdrawal. Histone acetylation promoter (Trichostatin A, TSA) and let-7f antagomir were applied during withdrawal. Mitochondrial respiration, let-7f level, and cell viability were assessed using XF respirometry, qPCR, TaqMan let-7f primers, and Calcein-AM assay, respectively. Repeated ethanol withdrawn rats show more than a twofold increase in histone acetylation, accompanied by mitochondrial respiratory suppression. EW-induced mitochondrial respiratory suppression is exacerbated by TSA treatment in a manner that is attenuated by let-7f antagomir cotreatment. TSA treatment does not alter the increasing effect of EW on let-7f level but dramatically exacerbates cell death induced by EW. These data suggest that the multiple episodes of withdrawal from chronic ethanol impede mitochondrial and cellular integrity through upregulating histone acetylation, independently or additively with let-7f.

PMID: 25406171 [PubMed - as supplied by publisher]

Coding ill-defined and unknown cause of death is 13 times more frequent in Denmark than in Finland

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Ylijoki-Sørensen, S. | Sajantila, A. | Lalu, K. | Bøggild, H. | Boldsen, J.L. | Boel, L.W.T.
Publication year: 2014
Journal / Book title: Forensic Science International

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Sigma-1 receptor stimulation protects retinal ganglion cells from ischemia-like insult through the activation of extracellular-signal-regulated kinases 1/2

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Mueller, B.H. | Park, Y. | Ma, H.-Y. | Dibas, A. | Ellis, D.Z. | Clark, A.F. | Yorio, T.
Publication year: 2014
Journal / Book title: Experimental Eye Research

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Autopsy rate in suicide is low among elderly in Denmark compared with Finland

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Ylijoki-Sørensen, S. | Boldsen, J.L. | Boel, L.W.T. | Bøggild, H. | Lalu, K. | Sajantila, A.
Publication year: 2014
Journal / Book title: Forensic Science International

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Resident Assistant Training Program for Increasing Alcohol, Other Drug, and Mental Health First-Aid Efforts

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Thombs, D.L. | Gonzalez, J.M.R. | Osborn, C.J. | Rossheim, M.E. | Suzuki, S.
Publication year: 2014
Journal / Book title: Prevention Science

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Methylene blue promotes quiescence of rat neural progenitor cells

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Xie, L. | Choudhury, G.R. | Wang, J. | Park, Y. | Liu, R. | Yuan, F. | Zhang, C.-L. | Yorio, T. | Jin, K. | Yang, S.-H.
Publication year: 2014
Journal / Book title: Frontiers in Cellular Neuroscience

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Discriminative and locomotor effects of five synthetic cathinones in rats and mice

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Gatch, M.B. | Rutledge, M.A. | Forster, M.J.
Publication year: 2014
Journal / Book title: Psychopharmacology

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Associations between time spent sitting and cancer-related biomarkers in postmenopausal women: an exploration of effect modifiers

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Paxton, R.J. | Jung, S.Y. | Vitolins, M.Z. | Fenton, J. | Paskett, E. | Pollak, M. | Hays-Grudo, J. | Hursting, S.D. | Chang, S.
Publication year: 2014
Journal / Book title: Cancer Causes and Control

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Cognitive Set Shifting Deficits and Their Relationship to Repetitive Behaviors in Autism Spectrum Disorder

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Miller, H.L. | Ragozzino, M.E. | Cook, E.H. | Sweeney, J.A. | Mosconi, M.W.
Publication year: 2014
Journal / Book title: Journal of Autism and Developmental Disorders

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Pregnancy Research on Osteopathic Manipulation Optimizing Treatment Effects: the PROMOTE study

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Hensel, K.L. | Buchanan, S. | Brown, S.K. | Rodriguez, M. | Cruser, d.A.
Publication year: 2014
Journal / Book title: American Journal of Obstetrics and Gynecology

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Using modified approaches on marginal regression analysis of longitudinal data with time-dependent covariates

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Zhou, Y. | Lefante, J. | Rice, J. | Chen, S.
Publication year: 2014
Journal / Book title: Statistics in Medicine

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Unsilencing voices: a study of zoo signs and their language of authority

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Fogelberg, K.
Publication year: 2014
Journal / Book title: Cultural Studies of Science Education

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Molecular genetic investigative leads to differentiate monozygotic twins

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Budowle, B.
Publication year: 2014
Journal / Book title: Investigative Genetics

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PTEN degradation after ischemic stroke: A double-edged sword

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Li, W. | Huang, R. | Chen, Z. | Yan, L.-J. | Simpkins, J.W. | Yang, S.-H.
Publication year: 2014
Journal / Book title: Neuroscience

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Characterization of [3H]LS-3-134, a novel arylamide phenylpiperazine D3 dopamine receptor selective radioligand

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Rangel-Barajas, C. | Malik, M. | Taylor, M. | Neve, K.A. | Mach, R.H. | Luedtke, R.R.
Publication year: 2014
Journal / Book title: Journal of Neurochemistry

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What Role Does Sleep Play in Weight Gain in the First Semester of University?

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Roane, B.M. | Seifer, R. | Sharkey, K.M. | Van Reen, E. | Bond, T.L.Y. | Raffray, T. | Carskadon, M.A.
Publication year: 2014
Journal / Book title: Behavioral Sleep Medicine

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Platinum(IV) cisplatin derivative trans, cis, cis-bis(heptanoato)amine(cyclohexylamine)dichloridoplatinum(IV) has an enhanced therapeutic index compared to cisplatin for the treatment of non-small cell lung cancer

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Shi, Y. | Koneru, B. | Redfearn, W. | Miller, M.L. | Myers, L. | Di Pasqua, A.J.
Publication year: 2014
Journal / Book title: Inorganica Chimica Acta

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A useful mouse model of glucocorticoid-induced ocular hypertension

Latest UNTHSC Articles in Scopus - 11 hours 49 min ago
Author(s):Clark, A.F.
Publication year: 2014
Journal / Book title: Investigative Ophthalmology and Visual Science

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